| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gao, Lindsay * (8)
|
OHLTPY |
46.47 |
42.44 |
-4.03 |
| 2 |
Wilkerson, Oliviah J (10)
|
OHLTPY |
45.69 |
45.00 |
-0.69 |
| 3 |
Erasmus, Madeleine D (10)
|
OHLTPY |
45.89 |
45.12 |
-0.77 |
| 4 |
Kelly, Alexa M (8)
|
OHLTPY |
46.30 |
46.24 |
-0.06 |
| 5 |
Brown, Morgan A (9)
|
OHLTPY |
49.24 |
48.90 |
-0.34 |
| 6 |
Shah, Aanya R (9)
|
OHLTPY |
54.33 |
49.74 |
-4.59 |
| 7 |
Johnston, Harper L (8)
|
OHLTPY |
57.59 |
51.91 |
-5.68 |
| 8 |
Rinker, Elliana G (10)
|
OHDRY |
48.00 |
53.06 |
5.06 |
| 9 |
Howell, Divya * (8)
|
OHLTPY |
|
53.45 |
- |
| 10 |
Neerukonda, Anika S (10)
|
OHLTPY |
54.37 |
54.25 |
-0.12 |
| 11 |
Smith, Leighton H (8)
|
OHLTPY |
53.15 |
55.52 |
2.37 |
| 12 |
Claggett, Aubrey V (5)
|
OHLTPY |
1:11.34 |
1:04.92 |
-6.42 |
| 13 |
Hayes, Ailee V (9)
|
OHLTPY |
1:02.70 |
1:06.41 |
3.71 |
| 14 |
McDonald, Penny J (5)
|
OHLTPY |
|
1:08.42 |
- |