| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gao, Sophia (10)
|
ADCPP |
35.22 |
34.21 |
-1.01 |
| 2 |
Hao, Helen Z (10)
|
ADCPP |
35.98 |
36.97 |
0.99 |
| 3 |
Waite, Kelly M (10)
|
ADDELM |
41.94 |
39.33 |
-2.61 |
| 4 |
Harpootlian, Hannah R (10)
|
ADCPP |
38.28 |
39.73 |
1.45 |
| 5 |
Carsillo, Ava C (9)
|
ADDELM |
40.72 |
40.84 |
0.12 |
| 6 |
Cronlund, Miranda M (10)
|
ADDELM |
43.45 |
43.88 |
0.43 |
| 7 |
Bourassa, Annalise (10)
|
ADCPP |
|
43.92 |
- |
| 8 |
Banik, Georgia H (10)
|
ADALBS |
46.73 |
45.76 |
-0.97 |
| 9 |
Raghuram, Samya (10)
|
ADALBS |
58.48 |
45.91 |
-12.57 |
| 10 |
Fronheiser, Addison K (10)
|
ADDELM |
43.84 |
47.10 |
3.26 |
| 11 |
Perri, Gabriella M (9)
|
ADDELM |
|
47.14 |
- |
| 12 |
Antolick, Ellen R (10)
|
ADALBS |
48.33 |
48.07 |
-0.26 |
| 13 |
Singh, Sonia (10)
|
ADCPP |
|
48.61 |
- |
| 14 |
Yafayeva, Roxana Z (10)
|
ADDELM |
43.75 |
49.03 |
5.28 |
| 15 |
Bray, Laila J (10)
|
ADALBS |
55.96 |
49.16 |
-6.80 |
| 16 |
Payamfar, Ranna (10)
|
ADALBS |
|
49.19 |
- |
| 17 |
Guilmette, Anna E (10)
|
ADALBS |
|
51.29 |
- |
| 18 |
Robles, Isabella M (9)
|
ADDELM |
1:07.15 |
59.46 |
-7.69 |
| 19 |
Dai, Chloe (9)
|
ADDELM |
|
1:04.14 |
- |
| 20 |
Bordell, Sophie R (10)
|
ADDELM |
|
1:07.60 |
- |
| 21 |
Hernandez, Ariana J (9)
|
ADDELM |
|
1:08.84 |
- |
| 22 |
Eliea-Shields, Madelyn R (10)
|
ADDELM |
|
1:09.15 |
- |
|
Farrell, Macayla M (9)
|
ADALBS |
|
DQ |
- |