| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kline, Keaghan E (10)
|
NESNAP |
34.32 |
34.44 |
0.12 |
| 2 |
Fronheiser, Addison K (10)
|
ADDELM |
41.13 |
41.74 |
0.61 |
| 3 |
Meyer, Penelope H (10)
|
ADDELM |
39.79 |
43.08 |
3.29 |
| 4 |
Dheeradhada, Arya J (9)
|
ADALBS |
|
43.30 |
- |
| 5 |
Banik, Georgia H (10)
|
ADALBS |
40.50 |
43.37 |
2.87 |
| 6 |
Perri, Gabriella M (10)
|
ADDELM |
41.35 |
44.27 |
2.92 |
| 7 |
Wolfort, Madison J (9)
|
ADALBS |
47.21 |
46.81 |
-0.40 |
| 8 |
Robles, Isabella M (10)
|
ADDELM |
48.51 |
47.50 |
-1.01 |
| 9 |
Allinson, Mae L (9)
|
ADDELM |
1:02.85 |
50.31 |
-12.54 |
| 16 |
Rajulu, Hasni K (10)
|
ADDELM |
|
1:03.59 |
- |
|
Farrell, Macayla M (9)
|
ADALBS |
|
DQ |
- |
|
Eliea-Shields, Madelyn R (10)
|
ADDELM |
1:06.39 |
DQ |
- |