| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Calhoun, Kyle (11)
|
IAOTT |
38.73 |
39.03 |
0.30 |
| 2 |
Kellenberger, Landon M (12)
|
IASCFY |
40.78 |
43.69 |
2.91 |
| 3 |
Saunders, Quinten (12)
|
IASCFY |
47.08 |
44.78 |
-2.30 |
| 4 |
Boettner, Kale (11)
|
IADUBQ |
46.74 |
45.09 |
-1.65 |
| 5 |
Stille, Isaac (11)
|
IADUBQ |
41.14 |
46.19 |
5.05 |
| 6 |
Paulsen, Benjamin (11)
|
IADUBQ |
51.27 |
49.83 |
-1.44 |
| 7 |
Wernimont, Will (11)
|
IADUBQ |
1:05.42 |
56.42 |
-9.00 |
| 8 |
Garcia-Lopez, Matthew (11)
|
IASCFY |
59.32 |
58.53 |
-0.79 |
| 9 |
Beasley, Braeden (11)
|
IADUBQ |
1:09.64 |
1:18.65 |
9.01 |
|
Manriquez, Cadance (11)
|
IADUBQ |
56.33 |
DQ |
- |