| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cartenuto, Katherine J (13)
|
NICLSC |
29.40 |
28.01 |
-1.39 |
| 2 |
Musterait, Mallory E (13)
|
NITWST |
29.64 |
28.16 |
-1.48 |
| 3 |
Machowski, Erin M (14)
|
NIHAMB |
27.43 |
29.20 |
1.77 |
| 4 |
Kubiak, Kelly A (14)
|
NITWST |
30.18 |
29.25 |
-0.93 |
| 5 |
Latona, Dena M (14)
|
NIHAMB |
28.65 |
29.42 |
0.77 |
| 6 |
Kwitek, Grace M (14)
|
NIEAST |
30.10 |
30.26 |
0.16 |
| 7 |
Kaiser, Grace (13)
|
NIAMTS |
32.68 |
31.10 |
-1.58 |
| 8 |
Berdysiak, Elizabeth C (13)
|
NICLSC |
31.08 |
31.59 |
0.51 |
| 9 |
Machowski, Stella J (14)
|
NITWST |
32.14 |
31.71 |
-0.43 |
| 10 |
Langlotz, Olivia M (14)
|
NICLSC |
33.05 |
31.74 |
-1.31 |
| 11 |
Keesler, Kaylynn N (14)
|
NIEMAC |
30.43 |
31.75 |
1.32 |
| 12 |
McKnight, Natalie (13)
|
NIAMTS |
32.12 |
32.42 |
0.30 |
| 13 |
Dzambegovic, Mia (13)
|
NIEAST |
|
49.69 |
- |
|
Sauer, Emma L (13)
|
NIHAMB |
28.53 |
27.81 |
-0.72 |
|
Barry, Caitlin E (14)
|
NITWST |
32.76 |
NS |
- |