| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Voit, Nicholas (10)
|
NIAMTS |
34.93 |
32.85 |
-2.08 |
| 2 |
Rudnicki, William S (10)
|
NITWST |
36.54 |
33.46 |
-3.08 |
| 3 |
Johnson, Landon J (9)
|
NIEMAC |
38.42 |
34.69 |
-3.73 |
| 4 |
Peresan, Brody J (10)
|
NIEAST |
35.34 |
34.71 |
-0.63 |
| 5 |
Cook, Bryce P (10)
|
NISWAT |
43.65 |
35.89 |
-7.76 |
| 6 |
Moldenhauer, Thomas C (9)
|
NIEAST |
36.00 |
35.91 |
-0.09 |
| 7 |
Liebenow, Miles R (10)
|
NIHAMB |
36.99 |
36.06 |
-0.93 |
| 8 |
Barone, Michael R (9)
|
NITWST |
41.07 |
36.84 |
-4.23 |
| 9 |
Keats, Brody J (10)
|
NITWST |
39.74 |
37.83 |
-1.91 |
| 10 |
Kwitek, Jack W (10)
|
NIEAST |
37.44 |
37.95 |
0.51 |
| 11 |
Sullivan, Nolan M (10)
|
NIHAMB |
41.07 |
38.07 |
-3.00 |
| 12 |
Delaney, Jack R (9)
|
NISWAT |
36.63 |
38.35 |
1.72 |
| 13 |
Smith, Jackson B (9)
|
NITWST |
45.36 |
39.14 |
-6.22 |
| 14 |
Gawron, Ian J (9)
|
NICLSC |
40.63 |
40.77 |
0.14 |
| 15 |
Krieger, Luke G (9)
|
NIEAST |
46.35 |
41.04 |
-5.31 |
| 16 |
Szymanski, Mason D (9)
|
NIEAST |
42.90 |
41.20 |
-1.70 |
| 17 |
Green, Archer W (10)
|
NIEAST |
41.11 |
41.81 |
0.70 |
| 18 |
Urbanek, Trenton J (9)
|
NICLSC |
42.38 |
42.62 |
0.24 |
| 19 |
Merrick, Emmett A (10)
|
NIEAST |
43.83 |
42.70 |
-1.13 |
| 20 |
Hart, Blake J (10)
|
NIUN-H |
|
43.33 |
- |
| 21 |
Scranton, Benjamin J (10)
|
NITWST |
48.67 |
43.80 |
-4.87 |
| 22 |
Johnson, Daniel P (9)
|
NITWST |
47.75 |
44.55 |
-3.20 |
| 23 |
LeGrano, Cannon B (9)
|
NIHHSC |
|
45.50 |
- |
| 24 |
Sauls, Clarke W (10)
|
NICLSC |
|
46.04 |
- |
| 25 |
Bibler, William S (9)
|
NICLSC |
|
49.62 |
- |
| 26 |
Bennett, Elijah H (9)
|
NIEAST |
52.99 |
53.98 |
0.99 |
| 27 |
Wallin, Alexander (9)
|
NITWST |
|
54.26 |
- |
|
Berdysiak, Lucas (9)
|
NITWST |
39.00 |
DQ |
- |
|
Densmore, Connor M (10)
|
NICLSC |
44.53 |
NS |
- |