| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Spence, Mackenzie (12)
|
COR |
30.14 |
30.19 |
0.05 |
| 2 |
Cheney, Brielle B (12)
|
MYST |
31.15 |
30.70 |
-0.45 |
| 3 |
Gracey-Dalton, Kendall L (12)
|
NIBRAD |
32.45 |
33.22 |
0.77 |
| 4 |
Chase, Bella (12)
|
COR |
33.41 |
33.73 |
0.32 |
| 5 |
Lucks, Summer (12)
|
WRN |
35.05 |
34.32 |
-0.73 |
| 5 |
Wilhelm, Morgan (12)
|
ZZESYS |
35.25 |
34.32 |
-0.93 |
| 7 |
Ball, Lauryn R (11)
|
NIBRAD |
41.21 |
34.39 |
-6.82 |
| 8 |
Quinn, Katherine E (12)
|
COUN |
34.95 |
34.45 |
-0.50 |
| 9 |
Lawry, Emily (12)
|
ZZESYS |
40.73 |
37.35 |
-3.38 |
| 10 |
Moats, Rachel (11)
|
COR |
40.02 |
37.81 |
-2.21 |
| 11 |
Faulk, Morgan (12)
|
YJET |
42.58 |
37.96 |
-4.62 |
| 12 |
Hutchinson, Bailey (11)
|
OCY |
39.91 |
38.02 |
-1.89 |
| 13 |
Bland, Skyler L (12)
|
MYST |
40.28 |
39.15 |
-1.13 |
| 14 |
Gindlesperger, Daphne C (12)
|
COUN |
39.04 |
39.49 |
0.45 |
| 15 |
Rospierski, Rachel (11)
|
ZZESYS |
|
39.60 |
- |
| 16 |
Bosco, Jenna M (12)
|
MYST |
38.51 |
39.82 |
1.31 |
| 17 |
Bish, Avery A (11)
|
MYST |
|
39.89 |
- |
| 18 |
Clark, Alijanayah (11)
|
YJET |
|
40.13 |
- |
| 19 |
Colwell, Sara (12)
|
COR |
39.14 |
41.55 |
2.41 |
| 20 |
Amon, Madison (11)
|
ZZESYS |
43.81 |
41.64 |
-2.17 |
| 21 |
Mattera, Kylie E (12)
|
MYST |
38.28 |
41.95 |
3.67 |
| 22 |
LaLone, Kathren (11)
|
TYST |
44.12 |
43.51 |
-0.61 |
| 23 |
Schmader, Korryn (11)
|
TYST |
39.37 |
43.73 |
4.36 |
| 24 |
McCullough, Bryndyn F (12)
|
MYST |
45.52 |
45.41 |
-0.11 |
| 25 |
Dvorak, Evabelle (12)
|
COUN |
46.20 |
47.61 |
1.41 |
| 26 |
Goray, Paige (11)
|
ZZESYS |
|
48.87 |
- |
| 27 |
Gummerson, Brynn (11)
|
ZZESYS |
59.04 |
51.08 |
-7.96 |
| 28 |
Sanders, Mia (11)
|
ZZESYS |
59.56 |
51.12 |
-8.44 |
| 29 |
Hanlon, Nyssa (12)
|
OCY |
55.06 |
57.41 |
2.35 |
| 30 |
Wyman, Crystal (11)
|
COR |
1:34.17 |
1:12.27 |
-21.90 |