| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cheney, Brielle B (12)
|
MYST |
34.37 |
34.85 |
0.48 |
| 2 |
Goodwill, Alli (12)
|
COR |
34.76 |
35.60 |
0.84 |
| 3 |
Moats, Taylor (12)
|
COR |
38.29 |
37.73 |
-0.56 |
| 4 |
Gracey-Dalton, Karsyn A (11)
|
NIBRAD |
38.33 |
37.91 |
-0.42 |
| 5 |
Quinn, Katherine E (12)
|
COUN |
38.88 |
39.38 |
0.50 |
| 6 |
Swain, Cassidy L (12)
|
COUN |
39.07 |
40.13 |
1.06 |
| 7 |
Borrero, Chloe (11)
|
ZZESYS |
39.35 |
40.19 |
0.84 |
| 8 |
Bidwell, Kylee (12)
|
WRN |
45.48 |
40.76 |
-4.72 |
| 9 |
Lucks, Summer (12)
|
WRN |
41.29 |
40.93 |
-0.36 |
| 10 |
Hart, Kelly (12)
|
ZZESYS |
42.34 |
41.13 |
-1.21 |
| 11 |
Lawry, Emily (12)
|
ZZESYS |
42.60 |
41.87 |
-0.73 |
| 12 |
Chase, Bella (12)
|
COR |
41.44 |
42.09 |
0.65 |
| 13 |
Crocker, Jenna (11)
|
TYST |
41.85 |
42.21 |
0.36 |
| 14 |
Colwell, Sara (12)
|
COR |
41.48 |
42.23 |
0.75 |
| 15 |
Newton, Lilly (11)
|
WRN |
42.00 |
42.32 |
0.32 |
| 16 |
Wilson, Grace (12)
|
COUN |
41.69 |
42.36 |
0.67 |
| 17 |
Hutchinson, Bailey (11)
|
OCY |
46.10 |
44.20 |
-1.90 |
| 18 |
Dvorak, Evabelle (12)
|
COUN |
43.60 |
45.02 |
1.42 |
| 19 |
Faulk, Morgan (12)
|
YJET |
43.58 |
45.13 |
1.55 |
| 20 |
Rospierski, Allison (12)
|
ZZESYS |
44.35 |
45.44 |
1.09 |
| 21 |
Burch, Chelsea (12)
|
ZZESYS |
46.84 |
45.45 |
-1.39 |
| 22 |
Medrick, Victoria P (12)
|
MYST |
43.96 |
45.46 |
1.50 |
| 23 |
Williams, Sabrina (12)
|
NIBRAD |
44.47 |
45.62 |
1.15 |
| 24 |
Bulger, Olivia (11)
|
ZZESYS |
47.93 |
45.73 |
-2.20 |
| 25 |
Bish, Avery A (11)
|
MYST |
|
45.88 |
- |
| 26 |
McCullough, Bryndyn F (12)
|
MYST |
46.59 |
46.00 |
-0.59 |
| 27 |
Hess, Ali (12)
|
COUN |
45.83 |
47.46 |
1.63 |
| 28 |
Dixon, Emilee (12)
|
OCY |
47.51 |
48.03 |
0.52 |
| 29 |
Waite, Leah (11)
|
YJET |
51.53 |
48.06 |
-3.47 |
| 30 |
Mong, Kallie (11)
|
OCY |
46.88 |
49.12 |
2.24 |
| 31 |
Campogiani, Adriana (12)
|
NIBRAD |
50.89 |
51.30 |
0.41 |
| 32 |
Hanlon, Nyssa (12)
|
OCY |
51.62 |
51.78 |
0.16 |
| 33 |
Clark, Alijanayah (11)
|
YJET |
58.90 |
53.69 |
-5.21 |
| 34 |
Mattera, Kylie E (12)
|
MYST |
51.31 |
54.03 |
2.72 |
| 35 |
Williams, Isabella L (11)
|
COUN |
53.00 |
54.93 |
1.93 |
| 36 |
Heyer, Gwen (12)
|
ZZESYS |
|
56.35 |
- |
| 37 |
Lindstrom, Kelly (12)
|
YJET |
1:02.45 |
1:01.73 |
-0.72 |
| 38 |
Wyman, Crystal (11)
|
COR |
|
1:13.33 |
- |
|
Pietrasiewicz, Alexis (11)
|
ZZESYS |
1:13.89 |
DQ |
- |
|
Sontheimer, Avery (11)
|
COR |
48.16 |
DQ |
- |
|
Hogan, Lily (11)
|
COR |
|
DQ |
- |
|
Wilhelm, Morgan (12)
|
ZZESYS |
44.55 |
NS |
- |