| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Golden, Elizabeth (12)
|
ZZESYS |
32.09 |
31.81 |
-0.28 |
| 2 |
Harrison, Lucy (11)
|
WRN |
34.69 |
33.46 |
-1.23 |
| 3 |
Gracey-Dalton, Karsyn A (12)
|
NIBRAD |
32.88 |
33.71 |
0.83 |
| 4 |
Mineo, Sydney (11)
|
COR |
33.23 |
34.50 |
1.27 |
| 5 |
Shade, Emma J (11)
|
COUN |
35.82 |
34.75 |
-1.07 |
| 6 |
Hoppe, Ellyse (12)
|
COUN |
35.72 |
34.84 |
-0.88 |
| 7 |
Goodwill, Mahra (11)
|
COR |
35.52 |
35.86 |
0.34 |
| 8 |
Maloney, Ava (11)
|
ZZESYS |
40.34 |
36.27 |
-4.07 |
| 9 |
Amon, Madison (12)
|
ZZESYS |
38.65 |
38.21 |
-0.44 |
| 10 |
Mitchell, Anna (12)
|
ZZESYS |
37.13 |
38.73 |
1.60 |
| 11 |
Sanders, Mia (12)
|
ZZESYS |
41.20 |
38.96 |
-2.24 |
| 12 |
Wilson, Ally (12)
|
FLY |
40.99 |
41.31 |
0.32 |
| 13 |
Risjan, Bailey (11)
|
COUN |
47.63 |
41.36 |
-6.27 |
| 14 |
Hren, Stella (11)
|
NIYJET |
39.87 |
42.17 |
2.30 |
| 15 |
Meyers, Emma (11)
|
ZZESYS |
41.97 |
42.40 |
0.43 |
| 16 |
Rihel, Reagyn (11)
|
ZZESYS |
39.71 |
42.68 |
2.97 |
| 17 |
Wilson, Kendall (11)
|
NIYJET |
49.49 |
47.64 |
-1.85 |
| 18 |
Sharrar, Annabella (11)
|
OCY |
|
49.79 |
- |
| 19 |
Lewis, Haylee (11)
|
OCY |
50.72 |
52.09 |
1.37 |
| 20 |
Hogan, Lily (12)
|
COR |
1:17.60 |
56.07 |
-21.53 |
| 21 |
Crofoot, June (11)
|
4MYST |
57.80 |
56.82 |
-0.98 |
|
Goray, Paige (12)
|
ZZESYS |
48.87 |
NS |
- |