| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brett, Erin E (11)
|
NIPACK |
45.85 |
47.34 |
1.49 |
| 2 |
Rossetti, Lena M (10)
|
NIPACK |
48.49 |
47.79 |
-0.70 |
| 3 |
Moroni, Brianna M (9)
|
NIIRON |
53.63 |
52.89 |
-0.74 |
| 4 |
McGahan, Molly E (9)
|
NIIRON |
54.52 |
52.91 |
-1.61 |
| 5 |
Viyannalage, Chloe A (10)
|
NIPACK |
54.65 |
54.34 |
-0.31 |
| 6 |
Brett, Catherine M (9)
|
NIPACK |
53.82 |
57.06 |
3.24 |
| 7 |
Hiller, Rachel J (8)
|
NIPACK |
1:04.17 |
58.07 |
-6.10 |
| 8 |
Besch-Turner, Juliet H (9)
|
NIFAST |
|
58.54 |
- |
| 9 |
Viyannalage, Riley A (9)
|
NIPACK |
|
58.77 |
- |
| 10 |
Grantham, Alliana R (9)
|
NIFAST |
1:07.00 |
1:05.66 |
-1.34 |
| 11 |
Sanders, Wendy I (9)
|
NIFAST |
|
1:08.16 |
- |
|
Dellis, Julia M (7)
|
NIPACK |
1:03.44 |
DQ |
- |