| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holcomb, Miyoko F (8)
|
ADSARY |
43.16 |
41.46 |
-1.70 |
| 2 |
Mulholland, Harper J (8)
|
ADSARY |
42.94 |
42.29 |
-0.65 |
| 3 |
Horn, Lucy A (8)
|
ADSCHE |
44.25 |
44.27 |
0.02 |
| 4 |
Lisai, Amelia S (8)
|
ADCPP |
54.84 |
45.53 |
-9.31 |
| 5 |
Perez, Mila V (8)
|
ADBFIN |
56.68 |
46.14 |
-10.54 |
| 6 |
Simmons, Carly M (7)
|
ADDELM |
|
49.53 |
- |
| 7 |
Stemper, Morgan E (8)
|
ADCPP |
|
49.81 |
- |
| 8 |
Pervychine, Elena N (8)
|
ADALBS |
|
51.59 |
- |
| 9 |
Skilbred, Laurel E (8)
|
ADDELM |
1:00.44 |
52.58 |
-7.86 |
| 10 |
Striker, Ella L (6)
|
ADCPP |
1:03.03 |
53.07 |
-9.96 |
| 11 |
Szlamowicz, Amy E (8)
|
ADCPP |
54.67 |
53.20 |
-1.47 |
| 12 |
Bobeldyk, Elsie M (7)
|
ADSARY |
51.62 |
54.87 |
3.25 |
| 13 |
Jayawardena, Devina N (7)
|
ADUSAM |
57.16 |
54.88 |
-2.28 |
| 14 |
Farinacci, Greta K (7)
|
ADBFIN |
|
56.78 |
- |
| 15 |
Carroll, Catherine J (7)
|
ADSARY |
58.81 |
57.59 |
-1.22 |
| 16 |
Keller, Madelyn G (8)
|
ADCPP |
1:06.28 |
57.73 |
-8.55 |
| 17 |
DeMartino, October A (7)
|
ADSCHE |
59.55 |
1:01.49 |
1.94 |
| 18 |
Smith, Avery E (7)
|
ADCPP |
|
1:01.94 |
- |
| 19 |
Hudson, Harper J (7)
|
ADSARY |
1:11.95 |
1:02.84 |
-9.11 |
| 20 |
Hu, Ruiqi R (7)
|
ADCPP |
1:08.58 |
1:03.09 |
-5.49 |
| 21 |
Constable, Mya C (7)
|
ADSARY |
1:06.08 |
1:03.95 |
-2.13 |
| 22 |
Kampf, Victoria J (8)
|
ADALBS |
|
1:04.77 |
- |
| 23 |
Freiberg, Bianca S (8)
|
ADDELM |
|
1:06.01 |
- |
| 24 |
Narang, Aarohi (8)
|
ADCPP |
1:14.07 |
1:06.78 |
-7.29 |
| 25 |
Yadav, Charvi (7)
|
ADCPP |
|
1:09.75 |
- |
| 26 |
Bubeck, Madison K (7)
|
ADCPP |
|
1:09.83 |
- |
| 27 |
Pollinger, Harper L (7)
|
ADDELM |
1:25.17 |
1:10.09 |
-15.08 |
| 28 |
Brown, Rowan F (8)
|
ADCPP |
|
1:12.56 |
- |
| 29 |
Gerace, Laurel A (8)
|
ADUSAM |
1:08.80 |
1:14.75 |
5.95 |
| 30 |
Sinclair, Alexandra C (7)
|
ADBFIN |
|
1:20.64 |
- |
| 31 |
Kraemer, Adelina L (7)
|
ADCPP |
|
1:24.22 |
- |
|
Wheeler, Amelia J (7)
|
ADBFIN |
|
DQ |
- |
|
Welch, Scarlett C (7)
|
ADALBS |
|
DQ |
- |