| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Coyner, Delaney K (8)
|
VANOVA |
43.87 |
42.78 |
-1.09 |
| 2 |
Patterson, Brantley A (8)
|
VANOVA |
47.49 |
47.92 |
0.43 |
| 3 |
Dodd, Margot B (8)
|
VASRVA |
52.10 |
48.04 |
-4.06 |
| 4 |
Bontrager, Peyton R (8)
|
VASRVA |
1:01.37 |
52.36 |
-9.01 |
| 5 |
Harvey, Parker J (8)
|
VANOVA |
49.67 |
52.94 |
3.27 |
| 6 |
Rolton, Francesca M (8)
|
VANOVA |
53.98 |
53.37 |
-0.61 |
| 7 |
Black, Samantha A (8)
|
VANOVA |
1:05.52 |
54.38 |
-11.14 |
| 8 |
Patterson, Mackenzie J (8)
|
VANOVA |
55.26 |
59.89 |
4.63 |
| 9 |
Hutton, Joy A (8)
|
VASRVA |
|
1:22.79 |
- |
| 10 |
McCormack, Isabel H (8)
|
VANOVA |
|
1:22.95 |
- |