| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lewis, Christopher J (9)
|
NIQUIK |
27.56 |
25.63 |
-1.93 |
| 2 |
Ritter, Madeleine (7)
|
NIPACK |
25.49 |
25.66 |
0.17 |
| 3 |
Frenzel, Jack W (9)
|
NIWEBS |
29.84 |
28.26 |
-1.58 |
| 4 |
McGahan, Molly E (7)
|
NIIRON |
29.59 |
31.00 |
1.41 |
| 4 |
Rene, Jax R (8)
|
NIIRON |
30.12 |
31.00 |
0.88 |
| 6 |
Yu, Emily X (8)
|
NIPACK |
30.61 |
31.05 |
0.44 |
| 7 |
Schaeber, Emerson M (9)
|
NIFAST |
34.18 |
32.53 |
-1.65 |
| 8 |
Brodt, Hannah C (9)
|
NIWEBS |
|
32.61 |
- |
| 9 |
Larzelere, Charlotte G (7)
|
NIFAST |
31.06 |
33.41 |
2.35 |
| 10 |
Dellis, Julia M (6)
|
NIPACK |
|
36.64 |
- |
| 11 |
Considine, Elizabeth A (8)
|
NIWEBS |
35.31 |
37.33 |
2.02 |
| 12 |
DiCaprio, James E (7)
|
NIFAST |
|
40.32 |
- |
| 13 |
Steele, Thomas J (7)
|
NIWEBS |
40.68 |
45.22 |
4.54 |
|
Grantham, Alliana R (7)
|
NIFAST |
|
DQ |
- |
|
Campanella, Jacob M (8)
|
NIWEBS |
|
DQ |
- |
|
Garigen, Molly G (5)
|
NIWEBS |
|
NS |
- |