| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wasserstrom, Abbie R (10)
|
MANSC |
45.91 |
45.02 |
-0.89 |
| 1 |
Murray, Claire E (10)
|
MAGAAC |
48.60 |
45.62 |
-2.98 |
| 2 |
Boles, Karly J (10)
|
MAGAAC |
45.22 |
45.39 |
0.17 |
| 2 |
DeCardona, Sophia E (9)
|
MAGAAC |
48.88 |
47.60 |
-1.28 |
| 3 |
Glynn, Delaney C (10)
|
MANSC |
44.94 |
46.14 |
1.20 |
| 3 |
Short, Sophia N (9)
|
MANSC |
48.98 |
48.04 |
-0.94 |
| 4 |
Mattice, Kenighan (10)
|
MALCY |
46.15 |
47.76 |
1.61 |
| 4 |
Brazer, Charlotte E (9)
|
MAGAAC |
51.52 |
49.61 |
-1.91 |
| 5 |
Soroka, Michelina R (10)
|
MADCAA |
49.84 |
49.74 |
-0.10 |
| 6 |
Kraft, Olivia G (9)
|
MADCAA |
48.05 |
50.06 |
2.01 |
| 7 |
Seidman, Jordan K (10)
|
MAGAAC |
52.21 |
50.18 |
-2.03 |
| 8 |
Lacovara, Leah (10)
|
MALCY |
51.21 |
50.47 |
-0.74 |
| 9 |
McFadden, Lucy G (10)
|
MACHEL |
51.68 |
51.74 |
0.06 |
| 10 |
Jones, Cooper (9)
|
MALCY |
49.70 |
52.09 |
2.39 |
| 11 |
Simonetti, Elsa B (9)
|
MACHEL |
54.20 |
53.56 |
-0.64 |
| 12 |
Ciechanowski, Leianna G (10)
|
MARAYS |
55.57 |
56.52 |
0.95 |
| 13 |
Commander, Abigail K (9)
|
MANHAC |
58.17 |
56.77 |
-1.40 |
| 14 |
Stensrud, Marin S (10)
|
MAGAAC |
1:05.37 |
58.77 |
-6.60 |
| 15 |
Winkelman, Gabrielle G (10)
|
NJTAC |
1:01.70 |
1:01.62 |
-0.08 |
| 16 |
Obenski, Lucille A (9)
|
MALCY |
1:04.96 |
1:07.32 |
2.36 |
|
Meyers, Sophia M (9)
|
MANSC |
55.41 |
NS |
- |
|
Coffman, Riley (10)
|
MALYM |
48.81 |
NS |
- |