| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Routzahn, Sophia M (13)
|
MDFAST |
2:24.78 |
2:26.24 |
1.46 |
| 2 |
Hendrickson, Margaret R (13)
|
MDEST |
2:31.18 |
2:27.14 |
-4.04 |
| 3 |
Daniel, Deborah J (14)
|
MDRAC |
2:34.00 |
2:27.66 |
-6.34 |
| 4 |
Arciaga, Savannah J (13)
|
MDGBSA |
2:30.03 |
2:31.16 |
1.13 |
| 5 |
Liu, Grace Z (13)
|
MDEST |
2:37.21 |
2:33.35 |
-3.86 |
| 6 |
Schmeckpeper, Emily D (13)
|
MDRAC |
2:29.54 |
2:36.40 |
6.86 |
| 7 |
Handwerk, Samantha K (14)
|
MDMSC |
2:37.10 |
2:40.59 |
3.49 |
| 8 |
Schwab, Hannah C (13)
|
MDGBSA |
2:46.33 |
2:46.01 |
-0.32 |
| 9 |
Mahle, Sophia G (13)
|
MDGBSA |
3:02.19 |
2:49.02 |
-13.17 |
| 10 |
Swallow, Penelope N (13)
|
MDGBSA |
3:20.91 |
2:59.87 |
-21.04 |
|
Farah, Nour J (13)
|
MDGBSA |
3:13.55 |
DQ |
- |