| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Asekun, Similoluwa A (11)
|
MDRAC |
2:34.64 |
2:35.24 |
0.60 |
| 2 |
von Wachter, Elizabeth C (11)
|
MDEST |
2:37.81 |
2:37.95 |
0.14 |
| 3 |
Jackson, Audrey L (12)
|
MDEST |
2:46.45 |
2:38.97 |
-7.48 |
| 4 |
Rodriguez, Mylah H (12)
|
MDEST |
2:48.39 |
2:40.63 |
-7.76 |
| 5 |
Kang, Jiyoon (12)
|
MDEST |
2:45.01 |
2:43.93 |
-1.08 |
| 6 |
Piggrem, Kadena L (12)
|
MDSPY |
2:49.38 |
2:45.36 |
-4.02 |
| 7 |
Birdsall, Kathleen S (12)
|
MDEST |
2:43.32 |
2:46.87 |
3.55 |
| 8 |
Johnson, Miya L (11)
|
MDFAST |
2:58.13 |
2:48.35 |
-9.78 |
| 9 |
Seung, Hannah M (11)
|
MDEST |
2:51.68 |
2:48.54 |
-3.14 |
| 10 |
Grey, Emily L (12)
|
MDASC |
2:54.62 |
2:48.59 |
-6.03 |
| 11 |
Azur, Eryn C (12)
|
MDEST |
2:51.41 |
2:50.80 |
-0.61 |
| 12 |
Joung, Ella D (11)
|
MDEST |
2:55.75 |
2:51.40 |
-4.35 |
| 13 |
Butler, Kyleigh G (12)
|
MDEST |
2:58.12 |
2:57.47 |
-0.65 |
|
Li, Erica Y (12)
|
MDEST |
2:46.05 |
DQ |
- |
|
Mauro, Josephine M (12)
|
MDCAA |
2:31.92 |
NS |
- |
|
O'Marra, Ivory R (12)
|
MDEST |
2:50.04 |
NS |
- |