| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fish, Shelby M (10)
|
MDCAA |
2:42.74 |
2:43.83 |
1.09 |
| 2 |
Firth, Kaitlyn L (10)
|
MDCAA |
2:54.51 |
2:48.39 |
-6.12 |
| 3 |
Freund, Gabrielle J (10)
|
MDCAA |
3:05.56 |
3:02.24 |
-3.32 |
| 4 |
Starr, Amelia J (9)
|
MDGBSA |
3:26.46 |
3:04.55 |
-21.91 |
| 5 |
Sanders, Koryn A (10)
|
MDCAA |
3:23.25 |
3:15.37 |
-7.88 |
| 6 |
Fitzsimmons, Christina M (9)
|
MDCAA |
3:33.84 |
3:18.27 |
-15.57 |
| 7 |
Wittholz, Katherine E (10)
|
MDCAA |
3:18.66 |
3:20.46 |
1.80 |
| 8 |
Harvey, Charlotte P (9)
|
MDGBSA |
3:47.04 |
3:36.02 |
-11.02 |
|
Ohtani, Mizuki (9)
|
MDCAA |
3:14.31 |
DQ |
- |
|
Vandewinckel, Olivia A (9)
|
MDGBSA |
3:39.09 |
DQ |
- |
|
Moozoun, Kianaaz (10)
|
MDCAA |
3:07.42 |
NS |
- |