| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Amrine, Elizabeth I (13)
|
OHLTPY |
33.77 |
35.05 |
1.28 |
| 2 |
Harrison-Mills, Mya L (13)
|
OHLTPY |
34.31 |
35.07 |
0.76 |
| 3 |
Lickfelt, Katie A (13)
|
OHLTPY |
39.94 |
39.87 |
-0.07 |
| 4 |
Blair, Cecilia J (13)
|
OHLTPY |
42.50 |
42.09 |
-0.41 |
| 5 |
Arehart, Gabrielle (14)
|
OHGCY |
42.91 |
44.04 |
1.13 |
| 6 |
Seth, Shivani * (13)
|
OHLTPY |
45.77 |
44.42 |
-1.35 |
| 7 |
Hayes, Olivia R (13)
|
OHLTPY |
45.17 |
47.59 |
2.42 |
| 8 |
Mohapatra, Subhra G (13)
|
OHLTPY |
49.86 |
48.71 |
-1.15 |
| 9 |
Riedl, Alyssa A (13)
|
OHLTPY |
|
49.63 |
- |
| 10 |
Osorno, Sara * (14)
|
OHLTPY |
|
50.68 |
- |
| 11 |
Uthup, Marissa * (13)
|
OHLTPY |
53.50 |
52.11 |
-1.39 |
| 12 |
Peruri, Tanvi P (13)
|
OHLTPY |
55.37 |
53.52 |
-1.85 |
|
Gudla, Sankeertana * (13)
|
OHLTPY |
50.69 |
NS |
- |