| # | Swimmer | Team | Seed | Time | Diff |
| 8 |
Bellissimo, Sophia G (14)
|
NCLOY |
29.96 |
28.59 |
-1.37 |
| 10 |
Claborn, Alyssa A (14)
|
NCLOY |
31.10 |
29.01 |
-2.09 |
| 21 |
McGuire, Molly S (13)
|
NCLOY |
34.91 |
31.03 |
-3.88 |
| 24 |
Seifert, Camille I (14)
|
NCLOY |
31.62 |
31.27 |
-0.35 |
| 26 |
Carpenter, Addison L (14)
|
NCLOY |
35.74 |
31.48 |
-4.26 |
| 27 |
Jones, Jessica E (14)
|
NCLOY |
|
31.88 |
- |
| 28 |
Murray, Kayla R (14)
|
NCLOY |
|
32.47 |
- |
| 32 |
Brochetti, Sophia F (13)
|
NCLOY |
34.56 |
32.87 |
-1.69 |
| 36 |
Wiechers, Abigail F (14)
|
NCLOY |
35.31 |
33.84 |
-1.47 |
| 37 |
Pechuekonis, Nikole M (13)
|
NCLOY |
37.05 |
33.96 |
-3.09 |