| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pierce, Camila E (16)
|
NCRSA |
2:25.19 |
2:24.90 |
-0.29 |
| 2 |
Bauer, Kaitlyn R (16)
|
VALY |
2:34.88 |
2:34.85 |
-0.03 |
| 3 |
Harrer, Sophia G (13)
|
NCRSA |
2:48.23 |
2:39.90 |
-8.33 |
| 4 |
McGonigal, Maeren R (15)
|
NCEAC |
2:35.00 |
2:42.80 |
7.80 |
| 5 |
Liu, Reina R (11)
|
NCGCY |
|
2:43.47 |
- |
| 6 |
Wooten, Hannah P (13)
|
NCRSA |
2:46.83 |
2:46.85 |
0.02 |
| 7 |
Lu, Angelina Z (13)
|
NCRSA |
2:51.09 |
2:51.00 |
-0.09 |
| 8 |
Serang, Lulu A (13)
|
NCTYDE |
2:48.08 |
2:52.67 |
4.59 |
| 9 |
High, Mary D (14)
|
NCTYDE |
2:53.94 |
2:57.51 |
3.57 |
| 10 |
Grant, Lindsay E (14)
|
NCRSA |
3:08.01 |
2:58.53 |
-9.48 |
| 11 |
Burleigh, Sydney R (13)
|
NCCKS |
3:20.27 |
3:02.71 |
-17.56 |
| 12 |
Trice, Zuri A (16)
|
NCCKS |
3:20.27 |
3:11.01 |
-9.26 |
| 13 |
Smith, Kelly M (17)
|
NCTYDE |
3:07.20 |
3:17.44 |
10.24 |
| 14 |
Sechriest, Makenzie R (14)
|
NCRACY |
|
3:47.84 |
- |
|
Alexander, Reece E (17)
|
NCTYDE |
2:29.52 |
NS |
- |
|
Jennings, Abby C (17)
|
NCTYDE |
|
NS |
- |
|
Rydzewski, Samantha G (15)
|
NCRSA |
2:33.44 |
NS |
- |
|
Rydzewski, Amber E (18)
|
NCRSA |
2:34.82 |
NS |
- |