| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stieber, Jack R (12)
|
NCTYDE |
36.01 |
34.36 |
-1.65 |
| 2 |
Golinski, Lukas S (12)
|
NCGCY |
40.35 |
36.71 |
-3.64 |
| 3 |
Cathey, Anderson M (12)
|
NCGCY |
35.73 |
37.25 |
1.52 |
| 4 |
Patterson, Carter J (11)
|
NCTYDE |
38.39 |
37.61 |
-0.78 |
| 5 |
Vogl, Joshua F (12)
|
NCTYDE |
38.00 |
38.06 |
0.06 |
| 7 |
Owens, Smith C (11)
|
NCWNCY |
39.53 |
41.65 |
2.12 |
| 8 |
Dodds, Wyatt H (12)
|
NCGCY |
44.16 |
43.49 |
-0.67 |
| 9 |
Yalcinkaya, Derin H (12)
|
NCTYDE |
43.73 |
45.38 |
1.65 |
| 11 |
O'Donnell, Colin J (11)
|
NCTYDE |
46.24 |
46.44 |
0.20 |
| 12 |
Weatherly, Keaton J (12)
|
NCRACY |
45.72 |
47.31 |
1.59 |
| 13 |
Dalton, Daniel J (11)
|
NCWNCY |
46.43 |
47.53 |
1.10 |
| 14 |
Jones, Alexander C (11)
|
NCGCY |
52.44 |
50.21 |
-2.23 |
| 15 |
Postle, Spencer (12)
|
NCTYDE |
|
50.26 |
- |
| 16 |
Hawkins III, Rodney D (11)
|
NCRACY |
54.94 |
56.23 |
1.29 |
| 16 |
Kohrt, Jason P (11)
|
NCTYDE |
55.59 |
56.23 |
0.64 |
| 18 |
Schofield, Ronald F (12)
|
NCRACY |
1:02.88 |
57.46 |
-5.42 |
| 19 |
Short, Timber A (11)
|
NCYSST |
|
57.96 |
- |
|
Funchess, Jr, Samuel W (11)
|
NCGCY |
|
DQ |
- |
|
Dettinger, Shane C (11)
|
NCGCY |
|
DQ |
- |
|
Abernathy, Harris S (12)
|
NCGCY |
36.35 |
NS |
- |
|
Wilson, William A (11)
|
NCGOLD |
1:45.87 |
NS |
- |