| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ross, Sadie B (8)
|
NCGCY |
|
20.44 |
- |
| 2 |
Petruzzi, Analise C (8)
|
NCTYDE |
24.04 |
21.60 |
-2.44 |
| 3 |
Urrea, Adrianna G (8)
|
NCTYDE |
23.22 |
23.39 |
0.17 |
| 4 |
Auer, Theresa G (8)
|
NCGCY |
32.75 |
24.52 |
-8.23 |
| 5 |
Olalere, Alexandra R (7)
|
NCGCY |
23.69 |
25.21 |
1.52 |
| 6 |
Crider, Kendyl A (8)
|
NCTYDE |
24.17 |
25.65 |
1.48 |
| 7 |
Gaylord, Lucy G (8)
|
NCGCY |
|
25.79 |
- |
| 8 |
Hawkins, Charlotte J (7)
|
NCRACY |
28.20 |
26.10 |
-2.10 |
| 10 |
Masneri, Olivia C (7)
|
NCGCY |
|
29.61 |
- |
| 11 |
Casale, Elsa S (8)
|
NCTYDE |
29.92 |
30.85 |
0.93 |
| 12 |
Goddard, Kyla J (8)
|
NCGCY |
28.67 |
30.92 |
2.25 |
| 13 |
Todd, Lilly K (6)
|
NCGCY |
29.37 |
32.07 |
2.70 |
| 14 |
Hall, Norah E (7)
|
NCTYDE |
28.58 |
32.36 |
3.78 |
|
Gann, Madison G (8)
|
NCGCY |
|
DQ |
- |
|
Henderson, Brooke C (8)
|
NCGCY |
|
DQ |
- |
|
Slaughter, Charlotte A (6)
|
NCGCY |
|
DQ |
- |