| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
George, Ruby M (12)
|
NCHCAC |
29.15 |
29.07 |
-0.08 |
| 2 |
Broadwell, Amelia L (11)
|
NCHCAC |
29.42 |
29.21 |
-0.21 |
| 3 |
Munzenmaier, Sarah A (12)
|
NCHCAC |
32.20 |
32.13 |
-0.07 |
| 4 |
Swenson, Emma E (12)
|
NCGOLD |
33.47 |
32.93 |
-0.54 |
| 5 |
Choate, Kalia'lani F (11)
|
NCFISH |
35.37 |
33.61 |
-1.76 |
| 6 |
Johnson, Caralina C (11)
|
NCBBS |
38.63 |
33.84 |
-4.79 |
| 7 |
Bissette, Avery L (11)
|
NCWBST |
32.58 |
33.89 |
1.31 |
| 8 |
Greene, Brooke E (11)
|
NCTRY |
34.03 |
33.92 |
-0.11 |
| 9 |
Brooks, Annica S (11)
|
NCHCAC |
40.61 |
35.63 |
-4.98 |
| 10 |
Prater, Mae-Lei (12)
|
NCHCAC |
39.55 |
36.20 |
-3.35 |
| 11 |
Jordan, Katherine G (11)
|
NCHCAC |
36.51 |
36.50 |
-0.01 |
| 12 |
Minter, Elena M (12)
|
NCWBST |
38.88 |
36.66 |
-2.22 |
| 13 |
Harper, Gabbrielle E (11)
|
NCGOLD |
37.78 |
37.32 |
-0.46 |
| 14 |
Kornegay, Linna B (11)
|
NCGOLD |
38.92 |
39.10 |
0.18 |
| 15 |
Williams, Caroline M (11)
|
NCCPDD |
39.90 |
40.49 |
0.59 |
| 16 |
Simpkins, Nora G (11)
|
NCBBS |
44.01 |
44.81 |
0.80 |
| 17 |
Dixon, Anna E (11)
|
NCGOLD |
49.58 |
45.25 |
-4.33 |
|
Hollowell, Cadence E (11)
|
NCFISH |
39.15 |
NS |
- |
|
Sagredo, Isabella (12)
|
NCCPDD |
41.96 |
NS |
- |