| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burckhardt, Ayden C (10)
|
NCHCAC |
40.50 |
41.28 |
0.78 |
| 2 |
Hardison, Eli C (10)
|
NCUN |
|
42.72 |
- |
| 3 |
Zou, Daniel (10)
|
NCGOLD |
53.89 |
45.18 |
-8.71 |
| 4 |
Johnson, Connor R (10)
|
NCGOLD |
45.96 |
45.22 |
-0.74 |
| 5 |
Bullard, Benjamin M (10)
|
NCUN |
|
47.00 |
- |
| 6 |
Horsman, Cody (10)
|
NCHCAC |
46.73 |
48.87 |
2.14 |
| 7 |
Stouffer, Joshua S (9)
|
NCGOLD |
43.42 |
49.56 |
6.14 |
| 8 |
Miller, Michael (10)
|
NCUN |
|
49.70 |
- |
| 9 |
Gribbin, Harry G (10)
|
NCGOLD |
52.89 |
50.48 |
-2.41 |
| 10 |
Pate, Ashton B (10)
|
NCGOLD |
|
1:29.82 |
- |
|
Chesnutt, Charles (10)
|
NCUN |
|
DQ |
- |
|
Coetzer, Eli C (10)
|
NCWBST |
55.06 |
DQ |
- |
|
Butterfield, Slade W (10)
|
NCGOLD |
|
NS |
- |