| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Packard, Samantha n (12)
|
NCTRY |
32.00 |
31.22 |
-0.78 |
| 2 |
Butler, Emily R (11)
|
NCHCAC |
33.80 |
34.13 |
0.33 |
| 3 |
Broadwell, Amelia L (11)
|
NCHCAC |
33.66 |
34.30 |
0.64 |
| 4 |
George, Ruby M (12)
|
NCHCAC |
34.18 |
35.71 |
1.53 |
| 5 |
Munzenmaier, Sarah A (12)
|
NCHCAC |
39.14 |
37.87 |
-1.27 |
| 6 |
Jordan, Katherine G (11)
|
NCHCAC |
39.31 |
39.53 |
0.22 |
| 7 |
Flanagan, Rianne L (11)
|
NCTRY |
39.53 |
41.36 |
1.83 |
| 8 |
Brooks, Annica S (11)
|
NCHCAC |
44.86 |
41.53 |
-3.33 |
| 9 |
Greene, Brooke E (11)
|
NCTRY |
44.33 |
41.69 |
-2.64 |
| 10 |
Prater, Mae-Lei (12)
|
NCHCAC |
51.56 |
42.51 |
-9.05 |
| 11 |
Kornegay, Linna B (11)
|
NCGOLD |
44.31 |
44.30 |
-0.01 |
| 12 |
Couter, Rubygail A (11)
|
NCWBST |
|
47.77 |
- |
| 13 |
Harper, Gabbrielle E (11)
|
NCGOLD |
46.17 |
48.32 |
2.15 |
|
Johnson, Caralina C (11)
|
NCBBS |
51.66 |
NS |
- |
|
Sagredo, Isabella (12)
|
NCCPDD |
|
NS |
- |