| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Butler, Emily R (11)
|
NCHCAC |
36.51 |
35.89 |
-0.62 |
| 2 |
Packard, Samantha n (12)
|
NCTRY |
36.78 |
37.58 |
0.80 |
| 3 |
Jordan, Katherine G (11)
|
NCHCAC |
42.19 |
41.75 |
-0.44 |
| 4 |
Flores, Stefanie (11)
|
NCUN |
|
43.02 |
- |
| 5 |
Munzenmaier, Sarah A (12)
|
NCHCAC |
43.34 |
43.96 |
0.62 |
| 6 |
Johnson, Caralina C (11)
|
NCBBS |
46.73 |
45.08 |
-1.65 |
| 7 |
Swenson, Emma E (12)
|
NCGOLD |
45.91 |
45.86 |
-0.05 |
| 8 |
Brooks, Annica S (11)
|
NCHCAC |
50.52 |
46.86 |
-3.66 |
| 9 |
Kornegay, Linna B (11)
|
NCGOLD |
47.93 |
46.96 |
-0.97 |
| 10 |
Prater, Mae-Lei (12)
|
NCHCAC |
52.00 |
52.02 |
0.02 |
| 11 |
Williams, Caroline M (11)
|
NCCPDD |
53.07 |
52.72 |
-0.35 |