| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harrington, Robert A (9)
|
NIGRAY |
46.22 |
43.88 |
-2.34 |
| 2 |
Sanderson, Billy L (10)
|
NIGRAY |
41.46 |
45.11 |
3.65 |
| 3 |
Cunningham, Antonio J (10)
|
NIGRAY |
44.98 |
46.80 |
1.82 |
| 4 |
Kelly, Gavin (10)
|
IHYS |
45.74 |
46.95 |
1.21 |
| 5 |
Savage, Kieran E (9)
|
NIGRAY |
|
47.31 |
- |
| 6 |
Singer, Evan (10)
|
NIGRAY |
46.98 |
47.61 |
0.63 |
| 7 |
Tellem, Aiden J (10)
|
NIGRAY |
43.53 |
49.59 |
6.06 |
| 8 |
Soler-Harand, Charles (10)
|
NIGRAY |
52.31 |
50.72 |
-1.59 |
| 9 |
Gonzalo Marin, Jose (10)
|
NIGRAY |
53.79 |
53.82 |
0.03 |
| 10 |
May, Devon (10)
|
ZZHRNY |
|
58.25 |
- |
|
Boulatnikov, Nicholas C (9)
|
NIGRAY |
58.14 |
DQ |
- |
|
Yip, Evan T (9)
|
NIGRAY |
|
DQ |
- |