| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fleck, Kristina S (8)
|
MDNBAC |
48.67 |
41.82 |
-6.85 |
| 2 |
Handwerger, Jenna E (8)
|
MDEST |
48.03 |
47.25 |
-0.78 |
| 3 |
Fisher, Chloe G (8)
|
MDNAAC |
47.42 |
48.31 |
0.89 |
| 4 |
McClure, Brynn A (8)
|
MDNAAC |
48.78 |
48.77 |
-0.01 |
| 5 |
Mo, Katina (8)
|
MDEST |
46.94 |
48.89 |
1.95 |
| 6 |
McGonigle, Saige M (8)
|
MDNAAC |
55.51 |
49.03 |
-6.48 |
| 7 |
Smith, Madison E (8)
|
MDEST |
48.34 |
50.43 |
2.09 |
| 8 |
Ponton-Scott, Ashira G (8)
|
MDBCSC |
57.82 |
53.71 |
-4.11 |
| 9 |
Stephan, Ainsley M (7)
|
MDEST |
50.69 |
54.05 |
3.36 |
| 10 |
You, Emily P (8)
|
MDEST |
50.76 |
55.99 |
5.23 |
| 11 |
Wu, Samantha W (8)
|
MDCAA |
|
1:03.13 |
- |
| 12 |
Kulynych-Irvin, Alders C (8)
|
MDNAAC |
|
1:07.46 |
- |
| 13 |
Agrawal, Sofya L (8)
|
MDCAA |
|
1:08.59 |
- |
| 14 |
Mahler, Madeline J (6)
|
MAHAC |
|
1:25.45 |
- |
|
Elliott, Aaliyah A (8)
|
MDUN-M |
49.81 |
58.03 |
8.22 |
|
Bergeron, Lillian E (8)
|
MDASC |
|
NS |
- |