| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hahn, Kurt C (12)
|
NISDSC |
34.56 |
34.60 |
0.04 |
| 2 |
Skizim, Elena K (11)
|
NISDSC |
38.41 |
37.75 |
-0.66 |
| 3 |
Tytler, Elizabeth (9)
|
NIIRON |
39.51 |
39.23 |
-0.28 |
| 4 |
Utz, Natalie R (12)
|
NISEAS |
44.91 |
44.14 |
-0.77 |
| 5 |
Bierley, Benjamin R (11)
|
NISDSC |
43.00 |
44.18 |
1.18 |
| 6 |
Kuhlkin, Elizabeth C (10)
|
NISEAS |
|
45.86 |
- |
| 7 |
Santmyer, Grace E (11)
|
NISDSC |
43.94 |
46.23 |
2.29 |
| 8 |
Robinson, Sara C (10)
|
NISDSC |
49.82 |
47.02 |
-2.80 |
| 9 |
Phillips, Hayley Q (9)
|
NIIRON |
52.16 |
47.11 |
-5.05 |
| 10 |
Maar, Stephanie M (11)
|
NISEAS |
46.05 |
47.25 |
1.20 |
| 11 |
Chen, Chloe Y (9)
|
NISDSC |
48.92 |
48.67 |
-0.25 |
| 12 |
Johnson, Ila A (11)
|
NIIRON |
|
48.78 |
- |
| 13 |
Kwiatkowski, Adam K (12)
|
NISEAS |
51.11 |
49.40 |
-1.71 |
| 14 |
Mattle, Skye M (10)
|
NISDSC |
47.36 |
49.66 |
2.30 |
| 15 |
Soto, Giovanni A (9)
|
NIIRON |
1:10.09 |
50.12 |
-19.97 |
| 16 |
Kelley, Gage T (9)
|
NISDSC |
49.88 |
50.36 |
0.48 |
| 17 |
Moroni, Brianna M (8)
|
NIIRON |
1:02.96 |
50.84 |
-12.12 |
| 18 |
Hyde, Keira E (10)
|
NIIRON |
47.47 |
51.63 |
4.16 |
| 20 |
Ashby, Jacob T (8)
|
NISDSC |
54.68 |
52.72 |
-1.96 |
| 21 |
Yaseen, Isam (9)
|
NIIRON |
|
53.45 |
- |
| 22 |
Miller, Ian M (7)
|
NIIRON |
|
53.72 |
- |
| 23 |
Miller, Kevin T (10)
|
NIIRON |
52.72 |
55.10 |
2.38 |
| 24 |
Hackett, Grace C (10)
|
NIIRON |
1:01.13 |
55.35 |
-5.78 |
| 25 |
Thoen, Sarah G (11)
|
NISDSC |
51.32 |
55.37 |
4.05 |
| 26 |
Ramsperger, Jacob T (9)
|
NISDSC |
57.94 |
59.60 |
1.66 |
| 27 |
Loewke, Abigail B (9)
|
NISEAS |
|
1:00.59 |
- |
| 28 |
Quinto, Lilian M (7)
|
NIIRON |
|
1:02.93 |
- |
|
Rene, Jax R (8)
|
NIIRON |
56.20 |
DQ |
- |
|
Pogroszewski, Frances S (9)
|
NISEAS |
47.03 |
NS |
- |