| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shen, Alivia (11)
|
NIPACK |
32.00 |
32.90 |
0.90 |
| 2 |
Sahin, Hazal (10)
|
NIPACK |
36.68 |
35.72 |
-0.96 |
| 3 |
Kunze, Sarah A (9)
|
NIPACK |
41.78 |
36.76 |
-5.02 |
| 4 |
Bosa, Alessandro N (9)
|
NIPACK |
48.37 |
44.85 |
-3.52 |
| 5 |
Stancampiano, Neel M (10)
|
NIPACK |
47.58 |
45.55 |
-2.03 |
| 6 |
Minnigh, Christina N (12)
|
NIPACK |
46.47 |
48.77 |
2.30 |
| 7 |
Gallant, Isabella A (9)
|
NIPACK |
49.13 |
48.83 |
-0.30 |
| 8 |
Brett, Erin E (10)
|
NIPACK |
46.34 |
50.33 |
3.99 |
| 9 |
Duran, Heidi K (9)
|
NIGRSC |
53.12 |
51.16 |
-1.96 |
| 10 |
Joshi, Nisha R (9)
|
NIPACK |
|
59.93 |
- |
| 11 |
Gonzalez, Alexis W (9)
|
NIPACK |
1:02.35 |
1:10.98 |
8.63 |
| 12 |
Belous, Matthew I (10)
|
NIPACK |
1:14.99 |
1:12.89 |
-2.10 |