| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Willis, Kylie E (10)
|
NIWEBS |
40.30 |
39.54 |
-0.76 |
| 2 |
Nitchman, Melina E (10)
|
NIWEBS |
50.18 |
50.83 |
0.65 |
| 3 |
Ignizio, Natalie V (10)
|
NIIRON |
|
52.75 |
- |
| 4 |
Masten, Olivia A (9)
|
NIWEBS |
|
55.04 |
- |
| 5 |
Mastrosimone, Nina A (9)
|
NIWEBS |
53.56 |
55.11 |
1.55 |
| 6 |
Pendry, Ella S (9)
|
NIFAST |
59.41 |
58.44 |
-0.97 |
| 7 |
Natale, Tessa T (10)
|
NIFAST |
1:03.15 |
1:06.90 |
3.75 |
| 8 |
Ricci, Lillian K (9)
|
NIWEBS |
1:05.75 |
1:14.94 |
9.19 |
|
Shaw, Fiona M (10)
|
NIFAST |
41.37 |
DQ |
- |
|
Brodt, Hannah C (9)
|
NIWEBS |
55.09 |
DQ |
- |
|
Galitsky, AnnaElizabeth (10)
|
NIGCOM |
|
NS |
- |
|
McCartney, Christina R (10)
|
NIGCOM |
|
NS |
- |