| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moroni, Brianna M (8)
|
NIIRON |
22.71 |
22.98 |
0.27 |
| 2 |
Kowal, Juliana G (8)
|
NIFAST |
|
24.56 |
- |
| 3 |
Rusho, Brynn J (6)
|
NIFAST |
28.83 |
27.17 |
-1.66 |
| 4 |
Mantell, Julia R (6)
|
NIIRON |
|
28.37 |
- |
| 5 |
McGrath, Gabrielle A (5)
|
NIFAST |
|
29.23 |
- |
| 6 |
Foote, Mackenzie G (6)
|
NIFAST |
|
30.95 |
- |
| 7 |
Rause, Gabby L (6)
|
NIFAST |
|
31.64 |
- |
| 8 |
Forsyth, Abigayle K (8)
|
NIIRON |
|
32.45 |
- |
| 9 |
Miller, Kylie M (6)
|
NIFAST |
31.97 |
33.23 |
1.26 |
| 10 |
Nacca, Nadia M (6)
|
NIFAST |
|
34.30 |
- |
| 11 |
Bernardi, Haven G (8)
|
NIIRON |
|
36.45 |
- |
| 12 |
Casella, Isabelle R (8)
|
NIFAST |
|
36.86 |
- |
| 13 |
Foote, Mallory E (8)
|
NIFAST |
|
38.76 |
- |
| 14 |
Behr, Gabriellia A (8)
|
NIFAST |
|
46.90 |
- |
|
Gucker, Jane E (5)
|
NIRRST |
|
DQ |
- |
|
Aintablian, Lillyanne R (8)
|
NIIRON |
|
NS |
- |