| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
O'Brien, Jolie L (10)
|
NIWEBS |
33.45 |
34.46 |
1.01 |
| 2 |
Mantell, Norah A (9)
|
NIIRON |
38.19 |
36.09 |
-2.10 |
| 3 |
Moroni, Brianna M (9)
|
NIIRON |
35.87 |
36.27 |
0.40 |
| 4 |
Mastrosimone, Nina A (10)
|
NIWEBS |
36.58 |
36.85 |
0.27 |
| 5 |
Yasin, Maram (9)
|
NIIRON |
42.30 |
38.04 |
-4.26 |
| 6 |
McGahan, Molly E (9)
|
NIIRON |
40.47 |
38.50 |
-1.97 |
| 7 |
Salisbury, Eden G (9)
|
NIWEBS |
44.65 |
38.53 |
-6.12 |
| 8 |
Quinto, Lilian M (9)
|
NIIRON |
39.04 |
39.35 |
0.31 |
| 9 |
Forsyth, Abigayle K (9)
|
NIIRON |
42.35 |
40.91 |
-1.44 |
| 10 |
Aintablian, Lillyanne R (9)
|
NIIRON |
50.41 |
42.70 |
-7.71 |
| 11 |
Schmidt, Casey N (10)
|
NIIRON |
|
43.78 |
- |
| 12 |
Tinnes, Natalie G (9)
|
NIWEBS |
|
47.94 |
- |
| 13 |
Herendeen, Rory E (10)
|
NIIRON |
|
51.29 |
- |
| 14 |
Hochreiter, Gretchen C (10)
|
NIWEBS |
57.17 |
55.29 |
-1.88 |
| 15 |
Zimmerman, Jayna H (9)
|
NIWEBS |
1:01.48 |
56.93 |
-4.55 |
|
Stasko, Cassidy A (9)
|
NIIRON |
1:06.74 |
NS |
- |