| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rene, Jax R (10)
|
NIIRON |
46.21 |
47.46 |
1.25 |
| 2 |
O'Brien, Luke P (9)
|
NIIRON |
50.72 |
51.16 |
0.44 |
| 3 |
Barnhart, Nicolas D (9)
|
NIWEBS |
52.46 |
51.47 |
-0.99 |
| 4 |
Giarrizzohoyle, Joseph B (10)
|
NIWEBS |
|
51.48 |
- |
| 5 |
Steele, Thomas J (9)
|
NIWEBS |
53.59 |
51.88 |
-1.71 |
| 6 |
Kaub, Jack B (10)
|
NIFAST |
56.37 |
54.77 |
-1.60 |
| 7 |
Muisus, Logan A (10)
|
NIWEBS |
56.20 |
55.16 |
-1.04 |
| 8 |
Schrack, Jacob A (9)
|
NIWEBS |
1:05.76 |
56.48 |
-9.28 |
| 9 |
Pellman, Jonathan P (9)
|
NIIRON |
|
59.43 |
- |
| 10 |
Madison, Owen P (10)
|
NIWEBS |
1:08.45 |
1:07.03 |
-1.42 |
|
Giarrizzohoyle, John A (10)
|
NIWEBS |
|
DQ |
- |
|
Cox, William K (10)
|
NIWEBS |
56.14 |
NS |
- |