| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McCutcheon, Chloe M (12)
|
NIAAAC |
2:25.56 |
2:26.49 |
0.93 |
| 2 |
Cadle, Mattison I (12)
|
NIPVSC |
2:36.21 |
2:28.45 |
-7.76 |
| 3 |
Harrington, Porsha M (12)
|
NIAAAC |
3:05.62 |
2:34.65 |
-30.97 |
| 4 |
Keesler, Kaylynn N (12)
|
NIEMAC |
2:49.23 |
2:42.04 |
-7.19 |
| 5 |
Coble, Autumn R (11)
|
NIGVST |
3:53.78 |
2:55.94 |
-57.84 |
| 6 |
Brandes, Tiernee S (11)
|
NIGVST |
3:03.37 |
2:57.46 |
-5.91 |
| 7 |
Jones, Sophia M (11)
|
NIPVSC |
3:09.53 |
3:14.22 |
4.69 |
| 8 |
Grusendorf, Adison E (11)
|
NIGVST |
|
3:17.53 |
- |
| 9 |
Haggstrom, Raygen M (11)
|
NIGVST |
|
3:26.91 |
- |
| 10 |
Fisher, Madalyn J (12)
|
NIPVSC |
4:14.63 |
3:49.21 |
-25.42 |