| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Liu, Reina R (8)
|
NCGCY |
39.15 |
36.52 |
-2.63 |
| 2 |
Zuleger, Skylar B (8)
|
NCTYDE |
39.16 |
40.32 |
1.16 |
| 3 |
Powell, Catherine G (8)
|
NCYOTA |
43.13 |
43.07 |
-0.06 |
| 5 |
Wehe, Eden J (8)
|
NCGCY |
46.28 |
46.77 |
0.49 |
| 6 |
Pace, Clementine S (8)
|
NCTYDE |
49.89 |
48.97 |
-0.92 |
| 7 |
Senter, Ainsley J (8)
|
NCCFAC |
59.40 |
51.70 |
-7.70 |
| 8 |
Herlocker, Patterson L (8)
|
NCRACY |
56.11 |
54.94 |
-1.17 |
| 10 |
Hinson, Alice-Kate M (8)
|
NCYOTA |
49.23 |
57.01 |
7.78 |
| 11 |
Dungan, Kenzington M (7)
|
NCYOTA |
1:07.28 |
1:00.21 |
-7.07 |
| 14 |
Houde, Victoria M (8)
|
NCYOTA |
1:12.88 |
1:10.17 |
-2.71 |
| 15 |
Maxwell, Harper D (8)
|
NCYOTA |
55.88 |
1:10.51 |
14.63 |
| 16 |
Heeden, Ashley G (7)
|
NCYOTA |
1:22.68 |
1:15.55 |
-7.13 |
| 17 |
Dailey, Gwendolyn P (7)
|
NCYOTA |
1:15.88 |
1:29.87 |
13.99 |
|
Chintapalli, Anjali P (7)
|
NCYOTA |
1:00.09 |
NS |
- |