| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Czirjak, Sara E (10)
|
VAGATR |
41.18 |
38.50 |
-2.68 |
| 2 |
Salerno, Isabella M (10)
|
VAGATR |
40.19 |
39.44 |
-0.75 |
| 3 |
Kestner, Lee R (10)
|
VAGATR |
40.97 |
40.13 |
-0.84 |
| 4 |
Ismailova, Diana (9)
|
VAGATR |
39.22 |
41.50 |
2.28 |
| 5 |
Wells, Sara T (10)
|
VAGATR |
42.06 |
43.66 |
1.60 |
| 6 |
Bradberry, Abigail L (10)
|
VAGATR |
46.44 |
45.45 |
-0.99 |
| 7 |
Murtaugh, Lindsay E (9)
|
VAGATR |
48.89 |
47.85 |
-1.04 |
| 8 |
Moody, Macy H (10)
|
VAGATR |
46.84 |
48.20 |
1.36 |
| 9 |
Sofi, Alishba (8)
|
VAGATR |
49.87 |
48.37 |
-1.50 |
| 10 |
Sylvester-Johnson, Jillian B (9)
|
VAGATR |
53.29 |
48.93 |
-4.36 |
| 11 |
Schaaf, Alexis V (9)
|
VAGATR |
53.05 |
49.46 |
-3.59 |
| 12 |
Jones, Cary E (9)
|
VASCAT |
59.55 |
52.43 |
-7.12 |
| 13 |
Shuler, Ella M (10)
|
VASCAT |
1:09.26 |
56.98 |
-12.28 |