| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kirtley, Miranda B (14)
|
VAGATR |
4:56.19 |
5:02.92 |
6.73 |
| 2 |
Isbell, Ruby T (12)
|
VAGATR |
5:19.49 |
5:28.18 |
8.69 |
| 3 |
Harris, Suzanne E (13)
|
VAGATR |
5:16.60 |
5:28.45 |
11.85 |
| 4 |
Summerlin, Caroline A (13)
|
VAGATR |
5:15.28 |
5:30.12 |
14.84 |
| 5 |
Eckert, Ellisa M (14)
|
VALY |
5:28.94 |
5:32.20 |
3.26 |
| 6 |
Murtaugh, Abigail N (14)
|
VAGATR |
5:29.04 |
5:32.26 |
3.22 |
| 7 |
Roberts, Alexis L (13)
|
VAGATR |
5:32.40 |
5:33.62 |
1.22 |
| 8 |
Turner, Caroline G (13)
|
VAGATR |
5:38.34 |
5:36.19 |
-2.15 |
| 9 |
Higgins, Clara E (12)
|
VAGATR |
5:31.41 |
5:45.96 |
14.55 |
| 10 |
Joseph, Emily S (13)
|
VAGATR |
5:40.05 |
5:47.02 |
6.97 |
| 11 |
Isbell, Lillian G (17)
|
VAGATR |
5:37.87 |
5:48.07 |
10.20 |
| 12 |
Whitlow, Lane O (11)
|
VAGATR |
5:48.90 |
5:51.38 |
2.48 |
| 13 |
Sofi, Liala (14)
|
VAGATR |
5:44.37 |
5:58.71 |
14.34 |
| 14 |
Sheffield, Haley S (11)
|
VASMAC |
6:09.69 |
6:00.52 |
-9.17 |
| 15 |
Wilcher, Ashlee L (11)
|
WVWILD |
6:01.82 |
6:01.49 |
-0.33 |
| 16 |
Scott, Alexa G (13)
|
VAGATR |
5:42.36 |
6:03.29 |
20.93 |
| 17 |
Stoner, Ava M (12)
|
VAGATR |
5:41.64 |
6:04.66 |
23.02 |
| 18 |
Wyatt, Elizabeth (13)
|
VAGATR |
6:03.46 |
6:05.26 |
1.80 |
| 19 |
Grove, Hannah C (15)
|
VASCAT |
6:51.97 |
6:14.92 |
-37.05 |
| 20 |
Bolling, Evelyn S (11)
|
VAGATR |
6:16.01 |
6:20.32 |
4.31 |
| 21 |
Russell, Caroline H (12)
|
VAGATR |
6:22.74 |
6:22.86 |
0.12 |
| 22 |
Peifer, Mia K (11)
|
VASMAC |
7:33.80 |
6:25.48 |
-68.32 |
| 23 |
Hill, Sydney G (11)
|
VASMAC |
6:33.08 |
6:31.26 |
-1.82 |
| 24 |
Mason, Brianna (9)
|
VASMAC |
7:04.36 |
6:53.44 |
-10.92 |
| 25 |
Warren, Grace K (12)
|
VALY |
7:21.58 |
6:56.08 |
-25.50 |
| 26 |
Recktenwald, Elizabeth J (13)
|
VASCAT |
8:00.00 |
7:29.49 |
-30.51 |