| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hewitt, James A (9)
|
VAGATR |
34.37 |
31.65 |
-2.72 |
| 2 |
Wolfe, Leviticus A (9)
|
VAGATR |
33.22 |
31.97 |
-1.25 |
| 3 |
McMahon, Robert W (10)
|
VASMAC |
36.63 |
33.32 |
-3.31 |
| 4 |
Weaber, Anderson E (9)
|
VASMAC |
35.15 |
34.11 |
-1.04 |
| 5 |
Bodenstein, Padraic J (10)
|
VAGATR |
38.09 |
34.35 |
-3.74 |
| 6 |
Smith, Sheldon M (10)
|
VAGATR |
35.72 |
34.48 |
-1.24 |
| 7 |
Oswald, Aiden C (10)
|
VASMAC |
36.37 |
34.51 |
-1.86 |
| 8 |
Giovannini, Niccolo J (9)
|
VAGATR |
35.97 |
36.25 |
0.28 |
| 9 |
Bubar, Caleb P (9)
|
VAGATR |
37.54 |
36.83 |
-0.71 |
| 10 |
Johnson, Alexander M (9)
|
VAGATR |
42.57 |
38.73 |
-3.84 |
| 11 |
Straight, Christian B (9)
|
VASMAC |
37.03 |
39.16 |
2.13 |
| 12 |
Fish, Xavier L (9)
|
VASMAC |
43.58 |
39.83 |
-3.75 |
| 13 |
McWhorter, Mason M (9)
|
VASMAC |
42.78 |
41.21 |
-1.57 |
| 14 |
Parrish, Brody M (10)
|
VAGATR |
55.31 |
50.49 |
-4.82 |