| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Markman, Leopold (12)
|
NIGRAY |
38.33 |
38.80 |
0.47 |
| 2 |
Van Quekelberg, Gage B (12)
|
NIGRAY |
42.27 |
40.90 |
-1.37 |
| 3 |
Singer, Jaden (12)
|
NIGRAY |
41.41 |
42.55 |
1.14 |
| 4 |
Laity, Canon (11)
|
CSAY |
47.03 |
43.92 |
-3.11 |
| 5 |
Angona, Joshua M (11)
|
NIGRAY |
44.90 |
48.15 |
3.25 |
| 6 |
Otero-Vazquez, Santiago (12)
|
NIGRAY |
59.94 |
49.78 |
-10.16 |
| 7 |
Zuo, Yiding (11)
|
NIGRAY |
|
53.66 |
- |
| 8 |
Williams, Oliver S (11)
|
NIGRAY |
|
55.24 |
- |
| 9 |
Zhang, Alexander Y (11)
|
NIGRAY |
|
56.49 |
- |
|
Lincoln, Zachary (12)
|
CSAY |
43.71 |
DQ |
- |
|
Tipple, Sean P (11)
|
NIGRAY |
54.91 |
DQ |
- |