| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boyer, Chloe G (10)
|
NIISWM |
2:40.37 |
2:36.93 |
-3.44 |
| 2 |
Kuznia, Arianna M (10)
|
NIJETS |
2:51.92 |
2:51.77 |
-0.15 |
| 3 |
Grosso, Addison G (10)
|
NIJETS |
2:54.34 |
2:52.31 |
-2.03 |
| 4 |
Szkotak, Caydence L (9)
|
NIBSSC |
3:03.32 |
3:06.74 |
3.42 |
| 5 |
Akley, Isabelle A (10)
|
NIJETS |
3:15.48 |
3:08.53 |
-6.95 |
| 6 |
Deal, Grace A (9)
|
ADCLIN |
|
3:10.43 |
- |
| 7 |
Gucciardi, Molly S (9)
|
NIJETS |
3:33.73 |
3:12.45 |
-21.28 |
| 8 |
Cohen, Evyn B (9)
|
ADCLIN |
|
3:14.38 |
- |
| 9 |
Gerges, Christina F (10)
|
NIJETS |
|
3:39.49 |
- |
| 10 |
Vavra, Addison E (10)
|
NIJETS |
|
4:04.25 |
- |
|
Pelnik-Wadsworth, Kaitryn M (10)
|
ADCLIN |
|
NS |
- |
|
Devendorf, Madelyn G (10)
|
NIJETS |
|
NS |
- |