| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ross, Jeffrey J (17)
|
NIJETS |
49.52 |
50.17 |
0.65 |
| 2 |
Lowe, William J (18)
|
NIUN-M |
51.65 |
50.51 |
-1.14 |
| 3 |
Merkling, Griffin W (14)
|
NIJETS |
54.06 |
52.90 |
-1.16 |
| 4 |
Yost, Anthony J (18)
|
NIMETS |
51.83 |
52.95 |
1.12 |
| 5 |
Bushnell, Christopher C (16)
|
NISYCH |
55.70 |
53.24 |
-2.46 |
| 6 |
Licciardello, John T (16)
|
NIJETS |
52.62 |
54.49 |
1.87 |
| 6 |
Schultz, Nicholas J (16)
|
NIJETS |
54.57 |
54.49 |
-0.08 |
| 8 |
Nguyen, Brandon T (15)
|
NIJETS |
54.70 |
54.56 |
-0.14 |
| 9 |
Long, Dylan M (16)
|
NIMETS |
53.61 |
55.05 |
1.44 |
| 10 |
Carr, Cameron M (16)
|
NISYCH |
55.24 |
55.88 |
0.64 |
| 11 |
Oad, Jonathan M (17)
|
NIBSSC |
54.80 |
57.39 |
2.59 |
| 12 |
Rosner, Solomon R (15)
|
NIMETS |
55.01 |
58.05 |
3.04 |
| 13 |
Bohman, Evan A (16)
|
NIBSSC |
57.58 |
59.90 |
2.32 |
| 14 |
Ocker, Joe M (15)
|
NIMETS |
59.32 |
1:01.30 |
1.98 |
| 15 |
Andrews, Alec J (12)
|
NIBSSC |
|
1:27.23 |
- |
| 16 |
Warner, Lucas M (7)
|
NIMETS |
1:52.84 |
1:45.62 |
-7.22 |
|
Richardson, Kyle A (17)
|
NIJETS |
55.99 |
NS |
- |
|
Griffin, Thomas D (17)
|
NIJETS |
47.86 |
NS |
- |
|
Thomas, Joshua D (16)
|
NIOLSC |
53.33 |
NS |
- |
|
Tague, William F (17)
|
NIISWM |
53.37 |
NS |
- |