| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rayburn, Myah A (10)
|
GUAQUA |
35.73 |
35.15 |
-0.58 |
| 2 |
Hadaegh, Kimiya L (10)
|
GUAQUA |
38.01 |
39.54 |
1.53 |
| 3 |
Schroeder, Madison A (10)
|
GUKATY |
39.96 |
39.89 |
-0.07 |
| 4 |
Hurtado, Sara M (10)
|
GUKATY |
39.63 |
40.77 |
1.14 |
| 5 |
Harms, Audrey H (9)
|
GUKATY |
40.30 |
41.22 |
0.92 |
| 6 |
Deffet, Danielle C (10)
|
GUKATY |
41.08 |
41.31 |
0.23 |
| 7 |
Freeman, Brooke E (10)
|
GUKATY |
41.11 |
41.39 |
0.28 |
| 8 |
Deffet, Cathryn E (10)
|
GUKATY |
40.40 |
42.09 |
1.69 |
| 9 |
Blackmon, Kyra L (9)
|
GUAMBU |
53.82 |
51.25 |
-2.57 |
| 10 |
Lavespere, Victoria L (9)
|
GUAMBU |
57.14 |
1:01.93 |
4.79 |
| 11 |
Heckendorn, Addy L (9)
|
GUAMBU |
1:05.71 |
1:04.68 |
-1.03 |
|
Stofberg, Kayla N (10)
|
GUKATY |
45.24 |
DQ |
- |