| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reitz, Meghan E (18)
|
NIHAST |
2:26.63 |
2:28.96 |
2.33 |
| 2 |
Stepanek, Livia G (15)
|
NIHAST |
2:31.39 |
2:33.25 |
1.86 |
| 3 |
Christodaro, Melina A (16)
|
NIHAST |
2:44.24 |
2:39.71 |
-4.53 |
| 4 |
Rich, Molly E (15)
|
NIHAST |
2:55.87 |
2:40.39 |
-15.48 |
| 5 |
Bonfiglio, Sara F (16)
|
NIGRSC |
2:43.05 |
2:44.17 |
1.12 |
| 6 |
Miller, Alayna E (13)
|
NIHAST |
2:43.19 |
2:44.23 |
1.04 |
| 7 |
Sahin, Melis (14)
|
NIPACK |
2:44.65 |
2:45.38 |
0.73 |
| 8 |
Amering, Sophie M (13)
|
NIHAST |
2:52.83 |
2:45.82 |
-7.01 |
| 9 |
Rees, Hannah E (13)
|
NIGRSC |
2:53.15 |
2:53.17 |
0.02 |
| 10 |
Quigley, Brienne K (13)
|
NIGRSC |
2:56.61 |
2:56.30 |
-0.31 |
| 11 |
Camman, Kylie L (14)
|
NIGRSC |
3:25.89 |
3:10.87 |
-15.02 |
| 12 |
Thompson, Daniella J (13)
|
NIGRSC |
|
3:55.77 |
- |