| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kidder, Elizabeth A (14)
|
NIHAST |
2:42.47 |
2:44.18 |
1.71 |
| 2 |
Bromage, Charlotte C (13)
|
NIQUIK |
3:00.43 |
3:00.37 |
-0.06 |
| 3 |
Sylvestri, Sofia I (13)
|
NIPACK |
2:56.15 |
3:00.62 |
4.47 |
| 4 |
Stepanek, Livia G (14)
|
NIHAST |
3:01.42 |
3:02.76 |
1.34 |
| 5 |
Reitz, Meghan E (17)
|
NIHAST |
2:56.98 |
3:03.65 |
6.67 |
| 6 |
Doughty, Emma J (15)
|
NIHAST |
3:00.69 |
3:06.29 |
5.60 |
| 7 |
Geoca, Zoey L (14)
|
NISDSC |
3:11.13 |
3:09.82 |
-1.31 |
| 8 |
VanHouten, Ellie T (13)
|
NIPACK |
3:19.13 |
3:11.97 |
-7.16 |
| 9 |
Evans, Mary A (12)
|
NIGRSC |
3:07.14 |
3:14.13 |
6.99 |
| 10 |
Murray, Margaret D (16)
|
NISDSC |
3:16.27 |
3:19.48 |
3.21 |
| 11 |
Sahin, Melis (13)
|
NIPACK |
3:15.93 |
3:19.50 |
3.57 |
| 12 |
Bonfiglio, Sara F (15)
|
NIGRSC |
3:13.94 |
3:20.68 |
6.74 |
| 13 |
Christodaro, Melina A (15)
|
NIHAST |
3:26.55 |
3:21.13 |
-5.42 |
| 14 |
Sifling, Malia D (13)
|
NIPACK |
3:18.02 |
3:21.91 |
3.89 |
| 15 |
Leslie, Jordyn A (14)
|
NIHAST |
3:38.69 |
3:23.50 |
-15.19 |
|
Kane, Emma L (15)
|
NIGRSC |
3:30.78 |
NS |
- |
|
Burns, Hannah B (14)
|
NIGRSC |
3:30.47 |
NS |
- |