| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rich, Molly E (15)
|
NIHAST |
2:57.16 |
2:56.84 |
-0.32 |
| 2 |
Reitz, Meghan E (18)
|
NIHAST |
2:56.98 |
2:59.40 |
2.42 |
| 3 |
Stepanek, Livia G (15)
|
NIHAST |
3:01.42 |
3:04.58 |
3.16 |
| 4 |
Amering, Sophie M (13)
|
NIHAST |
3:03.28 |
3:11.81 |
8.53 |
| 5 |
Bonfiglio, Sara F (16)
|
NIGRSC |
3:13.94 |
3:12.96 |
-0.98 |
| 6 |
Miller, Alayna E (13)
|
NIHAST |
3:22.32 |
3:14.42 |
-7.90 |
| 7 |
Sahin, Melis (14)
|
NIPACK |
3:15.93 |
3:15.21 |
-0.72 |
| 8 |
Christodaro, Melina A (16)
|
NIHAST |
3:21.13 |
3:17.82 |
-3.31 |
| 9 |
Rees, Hannah E (13)
|
NIGRSC |
3:19.80 |
3:20.57 |
0.77 |
| 10 |
Kennedy, Madaliene F (15)
|
NISDSC |
3:32.25 |
3:30.96 |
-1.29 |
| 11 |
Quigley, Brienne K (13)
|
NIGRSC |
|
3:40.81 |
- |
| 12 |
Christodaro, Raena M (13)
|
NIHAST |
|
3:41.13 |
- |
| 13 |
Camman, Kylie L (14)
|
NIGRSC |
|
3:45.92 |
- |