| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reitz, Meghan E (17)
|
NIHAST |
5:27.21 |
5:51.98 |
24.77 |
| 2 |
Kennedy, Madaliene F (14)
|
NISDSC |
6:00.12 |
6:03.10 |
2.98 |
| 3 |
Sylvestri, Sofia I (13)
|
NIPACK |
5:51.18 |
6:03.86 |
12.68 |
| 4 |
Geoca, Zoey L (14)
|
NISDSC |
5:52.12 |
6:09.39 |
17.27 |
| 5 |
Stepanek, Livia G (14)
|
NIHAST |
6:07.91 |
6:16.38 |
8.47 |
| 6 |
Doughty, Emma J (15)
|
NIHAST |
6:03.78 |
6:17.32 |
13.54 |
| 7 |
Bromage, Charlotte C (13)
|
NIQUIK |
6:20.31 |
6:17.64 |
-2.67 |
| 8 |
Christodaro, Melina A (15)
|
NIHAST |
6:32.77 |
6:29.20 |
-3.57 |
| 9 |
Sahin, Melis (13)
|
NIPACK |
6:33.52 |
6:29.56 |
-3.96 |
| 10 |
Leslie, Jordyn A (14)
|
NIHAST |
|
7:19.26 |
- |
| 11 |
Resch, Rebecka J (10)
|
NIGRSC |
|
8:10.48 |
- |
| 12 |
Greisch, Alisha D (10)
|
NIGRSC |
9:29.45 |
9:21.64 |
-7.81 |