| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brennan, Margaret (17)
|
NIPACK |
2:25.75 |
2:30.14 |
4.39 |
| 2 |
Stepanek, Livia G (15)
|
NIHAST |
2:34.55 |
2:32.98 |
-1.57 |
| 3 |
Reitz, Meghan E (18)
|
NIHAST |
2:23.35 |
2:33.10 |
9.75 |
| 4 |
Christodaro, Melina A (16)
|
NIHAST |
2:44.25 |
2:42.80 |
-1.45 |
| 5 |
Bonfiglio, Sara F (16)
|
NIGRSC |
2:41.46 |
2:42.98 |
1.52 |
| 6 |
Bouwens, Emma L (11)
|
NIHAST |
2:43.71 |
2:45.36 |
1.65 |
| 7 |
Rees, Hannah E (13)
|
NIGRSC |
2:50.68 |
2:46.78 |
-3.90 |
| 8 |
Miller, Alayna E (13)
|
NIHAST |
2:40.06 |
2:46.93 |
6.87 |
| 9 |
Quigley, Brienne K (13)
|
NIGRSC |
2:50.56 |
2:51.77 |
1.21 |
| 10 |
Amering, Sophie M (13)
|
NIHAST |
2:51.65 |
2:54.68 |
3.03 |
| 11 |
Smolinski, Evelynn J (11)
|
NIHAST |
3:30.73 |
3:01.12 |
-29.61 |
| 12 |
Davison, Asella M (15)
|
NISDSC |
3:04.80 |
3:05.03 |
0.23 |
| 13 |
Camman, Kylie L (14)
|
NIGRSC |
|
3:05.63 |
- |