| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kidder, Elizabeth A (15)
|
NIHAST |
2:16.42 |
2:18.67 |
2.25 |
| 2 |
Martin, Caroline E (17)
|
NIGCOM |
2:21.79 |
2:23.14 |
1.35 |
| 3 |
Reitz, Meghan E (18)
|
NIHAST |
2:23.35 |
2:26.21 |
2.86 |
| 4 |
Stepanek, Livia G (15)
|
NIHAST |
2:34.55 |
2:38.90 |
4.35 |
| 5 |
Miller, Alayna E (13)
|
NIHAST |
2:54.89 |
2:40.06 |
-14.83 |
| 6 |
Salonen, Amber (15)
|
NIGCOM |
2:33.56 |
2:43.30 |
9.74 |
| 7 |
Guzik, Tessa L (13)
|
NIGRSC |
2:43.81 |
2:43.45 |
-0.36 |
| 8 |
Salonen-Roperti, Marissa A (16)
|
NIGCOM |
2:36.94 |
2:46.94 |
10.00 |
| 9 |
Bouwens, Emma L (11)
|
NIHAST |
2:59.81 |
2:49.92 |
-9.89 |
| 10 |
Christodaro, Melina A (16)
|
NIHAST |
2:44.25 |
2:50.49 |
6.24 |
| 11 |
McDermott, Cleo K (13)
|
NISDSC |
2:45.72 |
2:50.92 |
5.20 |
| 12 |
Amering, Sophie M (13)
|
NIHAST |
|
2:51.65 |
- |
| 13 |
Robertson, Julia E (14)
|
NIRRST |
3:00.48 |
2:56.37 |
-4.11 |
| 14 |
Stepanek, Abbigail L (13)
|
NIHAST |
3:02.96 |
3:00.19 |
-2.77 |
| 15 |
Christodaro, Raena M (13)
|
NIHAST |
3:26.94 |
3:10.03 |
-16.91 |
| 16 |
Herron, Ella S (12)
|
NIHAST |
3:38.21 |
3:22.85 |
-15.36 |
|
Doughty, Emma C (13)
|
NISDSC |
2:57.26 |
NS |
- |
|
Larue, Jessie A (16)
|
NIUN-M |
|
NS |
- |