| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kelley, Michaela G (10)
|
NIUN-Q |
48.78 |
47.92 |
-0.86 |
| 2 |
Cafarelli, Brianna N (9)
|
NIUN-Q |
45.05 |
48.73 |
3.68 |
| 3 |
Manciocchi, Kayden M (8)
|
NIGRSC |
|
51.27 |
- |
| 4 |
Smolinski, Evelynn J (10)
|
NIHAST |
54.17 |
52.30 |
-1.87 |
| 5 |
Pogroszewski, Frances S (9)
|
NISEAS |
57.01 |
54.17 |
-2.84 |
| 6 |
Bouwens, Emma L (10)
|
NIHAST |
56.25 |
55.93 |
-0.32 |
| 7 |
Schwandt, Natalie K (9)
|
NIGRAT |
59.99 |
57.31 |
-2.68 |
| 8 |
Anthony, Jillian M (9)
|
NIPMSC |
58.73 |
59.08 |
0.35 |
| 9 |
Beghini, Sofia A (9)
|
NIHAST |
1:12.49 |
1:00.73 |
-11.76 |
| 10 |
Orlando, Lillian G (8)
|
NIPACK |
|
1:02.89 |
- |
| 11 |
Crowley, Lila M (8)
|
NIGRSC |
1:11.33 |
1:06.92 |
-4.41 |
| 12 |
Rist, Olivia B (10)
|
NIHAST |
1:05.38 |
1:07.35 |
1.97 |
| 13 |
Strausser, Cadence A (9)
|
NIGRAT |
1:11.69 |
1:14.64 |
2.95 |
|
Maibohm, Brean R (10)
|
NIHAST |
|
DQ |
- |
|
Kemp, Abigail D (9)
|
NIHAST |
1:22.47 |
NS |
- |